[v4.1, v3.2] SnapGene – Industry’s most popular molecular cloning Tool

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It is very important for students, researchers, or biomedical practitioners in the field of molecular biology to have a professional and easy-to-use tool for DNA sequence analysis of biomolecules. Thereinto, SnapGene provides users with professional biological analysis functions; helps users quickly plan, visualize and record the biological analysis procedure; provides a safer and faster approach; and provides a lot of help for daily molecular biology learning and research.

As a powerful and versatile tool for molecular biology on Windows and Mac platforms, SnapGene helps user analyze all kinds of detailed data about DNA; creates and modifies DNA maps; compares DNA data; generates detailed DNA sequence files; comes with cloning technology for creating seamless gene fusion; and provides a variety of functions that biological researchers need at work.

SnapGene allows user to visualize and simulate what they’re doing with DNA, and intelligently alerts the user if an error occurs. All the operations done by users on DNA will be recorded in the software. Besides, SnapGene also provides detailed technical working principles for users, helping them conduct more convenient and intelligent operations in study and work.

// Key Features //

Feature Description
Molecular Cloning
  • Restriction Cloning
  • Gateway Cloning
  • Gibson Assembly
  • NEBuilder HiFi Assembly
  • In-Fusion Cloning
  • TA & GC Cloning
  • TOPO Cloning
  • Design primers
  • Anneal two oligos to form a double-stranded product
PCR and Mutagenesis
  • Simulate PCR
  • Overlap extension PCR
  • Primer-directed mutagenesis
Enzyme Sets
  • Predefined enzyme sets – by company or cutter
  • Create custom enzyme sets
  • View detailed enzyme information
  • Rich support for methylation sensitivity and associated error prevention
Convert File Formats
  • Alignment Formats
  • ApE
  • CLC Bio
  • Clone Manager
  • DNA Strider
  • DNADynamo
  • DNAssist
  • DNASTAR Lasergene
  • DS Gene
  • EMBL (ENA)
  • EnzymeX
  • GenBank / DDBJ
  • Gene Construction Kit
  • Geneious
  • GeneTool
  • Genome Compiler
  • Jellyfish
  • MacVector
  • pDRAW32
  • Serial Cloner
  • Swiss-Prot
  • Vector NTI
  • Visual Cloning
Agarose Gel Simulation
  • Simulate an agarose gel
  • Simulate a Restriction Digest
  • Simulate a PCR Amplification
  • Large collection of MW markers
Features / Annotations
  • Create and edit features
  • Automatic annotation of common features
  • Annotate novel features manually
  • Choose Alternative Codons
  • Sophisticated numbering of feature translations
  • Support for ribosomal slippage
  • View and edit translated features
  • Open reading frames (ORFs)
  • Whole-sequence translations
  • Check reading frames for gene fusions
  • Make Protein (from DNA)
  • Reverse Translate (from Protein)
  • Align DNA sequences with a reference sequence
  • Verify cloning or mutagenesis
  • Align cDNA to a chromosome
  • Pairwise and multi sequence DNA and Protein alignment
  • Choice of alignment algorithms – Clustal Omega, MAFFT, MUSCLE, T-Coffee
  • Contig Assembly
  • See multiple views of a DNA sequence
  • Large sequence support – browse chromosome size sequences
  • Edit DNA and protein sequences
  • Color code sequences
History Tracking
  • Comprehensive “undo” capability
  • See a graphical history of a product
  • Use optional history colors to identify the most recent change to a sequence
Data Management
  • Import from common file formats including annotations and notes
  • Export to standard formats
  • Create and share Collections
  • Share data with SnapGene Viewer
  • Run batch operations
  • Search for DNA or protein sequences
  • Search for enzymes, features or primers
General Cross platform compatibility – Windows, macOS, Linux

// Official Demo Video //

// Edition Statement //

AppNee provides the SnapGene Corporate Edition multilingual full installers, unlocked files and universal unlock patch for Windows 32-bit and 64-bit, and Mac.

// Installation Notes //

for v3.2.1, v4.1.9 on Windows:

  1. Download and install program, do not run
  2. Copy the unlock patch to installation folder, and run it
  3. Input your desired name, click ‘Patch And Register‘, and click ‘Yes
  4. Copy the unlocked file to installation folder and overwrite
  5. Done

*** This patch and method also work for v5.x.x, but that has some bugs, like: automatically exits program when you edit the DNA sequence; the DNA sequence disappears when you scroll the page…

// Prompts //

  • Delete the following folder can initialize the program:
    • C:\Users\AppNee.com\AppData\Roaming\GSLBiotech

// Warning //

The unlock patch might be reported as  threat Win32:Rootkit-gen [Rtk] with high risk. Use VirusTotal to make a full, authoritative scan, and decide to use it or not by yourself.

*** Please remember: antivirus software is not always right, just be careful and use it at your own risk.

// Related Links //

// Download URLs //

License Version Download Size
for Windows
Corporate Edition v3.2.1 32-bit 32.9 MB
v4.1.9 32-bit 40.2 MB
v5.3.1 failed n/a
for Mac
Corporate Edition v3.2.1 43.1 MB


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